Journal: Integrative biology : quantitative biosciences from nano to macro
Article Title: Vascular Smooth Muscle Contractility Depends on Cell Shape
doi: 10.1039/c1ib00061f
Figure Lengend Snippet: Microcontact printing used to provide spatial cues for vascular tissue self-organization. (A) Phase contrast images of VSMCs micropatterned into thin tissue lines of varying widths, indicated in each image, separated by 100 μm gaps. (scale bars =200 μm). (B) 3-D confocal image of 100 μm wide patterned VSMC tissue. (Green: f-actin, Blue: nuclei). Scale bar 100 μm. (C) Thickness map of tissue in B, using f-actin staining. (D) Tissue thickness as a function of pattern width and ECM (fibronectin (FN) and laminin (LN)) substrate (mean +/− SD).
Article Snippet: Human umbilical artery vascular smooth muscle cells (VSMCs) were purchased from Lonza (Walkersville, MD) at passage 3 and cultured in growth medium consisting of M199 culture medium (GIBCO, Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Invitrogen), 10 mM HEPES (GIBCO, Invitrogen, Carlsbad, CA), 3.5 g/L glucose, 2mg/L vitamin B-12, 50 U/ml penicillin and 50 U/mL streptomycin (GIBCO).
Techniques: Staining